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KMID : 0903619970380040372
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Journal of the Korean Society for Horticultural Science
1997 Volume.38 No. 4 p.372 ~ p.378
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Effect of Number of Days from Pollination to Detachment of Ovules on Embryo Development in Vitro for the Interspecific Crossing between Brassica Species and Intergeneric Hybridization between Brassica and Raphanus
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Abstract
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Interspecific and intergeneric hybridization involving more than 110 cross combinations between genus Brassica and Raphanus were performed. Ovules resulted from artificially pollinated flowers were cultured on MS basal medium, and the effect of number of days from pollination to ovule detachment on embryo development through the ovule culture was investigated. Embryo development from ovule cultured on MS media was relatively high when the ovule was taken 10 to 15 days after pollination, with some discrepancies depending on cross combinations. Optimum sucrose concentration was 5% for embryo rescue. Differences in embryo development from ovule culture depended on year of hybridization with some cross combinations. Use of ´Daibyosobudori´ as male parent resulted in low embryo development. Within the same species, cultivar differences in obtaining successful hybrids were observed.
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